dUTP incorporation into genomic DNA is linked to transcription in yeast

Abundant ribonucleotide incorporation into DNA by yeast replicative polymerases.

Measurements of nucleoside triphosphate levels in Saccharomyces cerevisiae reveal that the four rNTPs are in 36- to 190-fold molar excess over their corresponding dNTPs. During DNA synthesis in vitro using the physiological nucleoside triphosphate concentrations, yeast DNA polymerase epsilon, which is implicated in leading strand replication, incorporates one rNMP for every 1,250 dNMPs. Pol del...

Identification of DNA Markers Linked to Blooming Time in Almond

In this study flowering time and other important morphologic traits were evaluated during two years in F1 almond progenies of seventy two seedlings from cross between the intermediate flowering Italian cultivar ‘Tuono’ and the extra-late blooming Iranian cultivar ‘Shahrood-12’. A modified-bulk segregant analysis in combination with the application of the 140 RAPD primers, 87 nuclear ...

Recombination between DNA repeats in yeast hpr 1 Δ cells is linked to transcription elongation RNA polymerase

ation (Nickoloff and Reynolds, 1990; Nickoloff, 1992) The induction of recombination by transcription activand gene targeting (Thyagarajan et al., 1995) in mamation has been documented in prokaryotes and eukarymalian cells, and site-specific recombination in the otes. Unwinding of the DNA duplex, disruption of immunoglobulin genes (Blackwell et al., 1986; Leung and chromatin structure or change...

Mechanisms Employed by Escherichia coli to Prevent Ribonucleotide Incorporation into Genomic DNA by Pol V

Escherichia coli pol V (UmuD'(2)C), the main translesion DNA polymerase, ensures continued nascent strand extension when the cellular replicase is blocked by unrepaired DNA lesions. Pol V is characterized by low sugar selectivity, which can be further reduced by a Y11A "steric-gate" substitution in UmuC that enables pol V to preferentially incorporate rNTPs over dNTPs in vitro. Despite efficien...

Ara-C incorporation into DNA.